Giving a midstream urine sample

  • You can obtain a sterile container and vacuum test tubes for a urine sample from the lab or purchase them at a pharmacy. It is not allowed to use any other containers.
  • Do not touch the inside of the container or place anything in it before giving a sample.
  • Urinate directly into the container. It is not allowed to pour urine from one container into another.
  • Use the first urine in the morning (first-void urine) for the sample.
  • Do not eat or drink 10–14 hours before giving a urine sample. If needed, you may drink up to one cup of pure unflavoured still water during this time.
  • The urine must have stayed in your bladder 4–6 hours before giving a sample, meaning that at least 4–6 hours must have passed from the previous urination.
  1. Before giving a urine sample, wash your external genitalia with warm running water. Do no use any soap, sponge, etc. It is important to wash the external urethral orifice. Women should spread the labia with the fingers and men should pull back the foreskin when washing.
  2. Dry the external genitalia gently with a paper towel.
  3. Make sure the external urethral orifice is uncovered when giving a sample.
  4. Women having vaginal discharge or menstrual blood should use a tampon.
  5. To give a sample, remove the cap of the container and place it within reach, sticker beneath, without touching the inner surface of the container. NB! Do not remove the sticker from the cap of the container!
  6. To give a sample, begin urinating into the toilet. After urinating a few millilitres, without interrupting the flow of urine, collect the urine into the container and fill about 3/4 of the container. Finish urinating into the toilet.
  7. Close the container containing the sample tightly with the cap.
  8. Transfer the urine from the container into the test tube within 15 minutes of giving the sample.
    • NB! Do not remove the caps from the test tubes!
    • If you need to use various test tubes, fill the tube with the green cap first and then the one with the beige cap.
    • To transfer urine into a test tube:
      • Remove the protective sticker from the test tube cap partially (do not throw it away) and you will see a rubber-coated needle.
      • Press the test tube (cap end first) to the bottom of the opening in the cap of the urine container. Hold the test tube until the flow of urine into the tube has stopped. Remove the test tube from the opening and, if needed, fill the other test tube. Once the test tubes are full, re-seal the opening with the sticker.
      • Gently turn the test tubes bottom up 8–10 times so that the preservative contained in the tube can dissolve.
  9. Mark the test tube(s): write your name on the test tube or stick the bar code vertically from top to bottom, as straight and smooth as possible, and mark the date and time of taking the sample.
  10. Used sample container:
    • Check that the opening in the cap of the sample container is properly sealed with the sticker.
    • Pour any remaining urine from the container into the toilet.
    • Dispose of the container with household waste.
  11. Take the samples to the lab the same day.
  12. Until you take the samples to the lab, keep them in the refrigerator (at +2…+8°C).
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